Figure 2.

Role of Ca²⁺ channels in flow‐stimulated endothelin‐1 (ET‐1) mRNA levels in MPK‐CCD cells. Cells were preincubated with vehicle, 20 μmol/L SKF96265, 3 μmol/L BTP2, 10 μmol/L Pyr3, 30 μmol/L RN1734, 10 μmol/L lanthanum, or 10 μmol/L gadolinium for 30 min, followed by exposure to static conditions or shear stress at 2 dyne/cm² for 2 h in the presence of the same agents, then determination of ET‐1/GAPDH mRNA levels. N = 8–12 each data point. *P < 0.05 versus baseline (vehicle alone) no flow conditions; **P < 0.05 versus baseline no flow conditions and versus baseline flow conditions.