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Role of purinergic agents on flow‐stimulated endothelin‐1 (ET‐1) mRNA levels in MPK‐CCD cells. Cells were preincubated with vehicle, 30 μmol/L ATPγS, or 30 μmol/L PPADS for 30 min, followed by exposure to static conditions or shear stress at 2 dyne/cm2 for 2 h in the presence of the same agents, then determination of ET‐1/GAPDH mRNA levels. N = 8–12 each data point. *P < 0.05 versus baseline (vehicle alone) no flow conditions.
