Figure 2.

CXCL12 overexpression modifies the endogenous antioxidant enzymes’ activities and protein levels in Rin-5F and islet cells in basal and oxidative stress conditions. (A) CuZnSOD, MnSOD and CAT enzyme activities in pancreatic cells. (B) Relative protein levels of CuZnSOD, MnSOD and CAT in pancreatic cells estimated by immunoblot analysis of homogenates. Representative blots from three independent experiments are shown. The results of quantification of the immunoreactive bands relative to β-actin loading controls are presented in the graphs. wt – wild-type Rin-5F; empty vector-transfected Rin-5F – mock; #1 – CXCL12 overexpressing Rin-5F; (+) and (−) – islet cells with or without pretreatment with recombinant CXCL12 (80 ng/ml; 30 min). C – control; T1h – H₂O₂ (IC₅₀) treated for 1 h. T1h/12h – H₂O₂ (IC₅₀) treatment for 1 h followed by 12 h recovery. Results are expressed as means ± SEM. Means not sharing a common letter are significantly different between groups (p < 0.05).