Skip to main content
. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Biomed Mater Res A. 2017 Feb 2;105(4):1123–1131. doi: 10.1002/jbm.a.36008

Figure 1.

Figure 1

Coculture in Alginate and Collagen Scaffolds. A) Experimental setup depicts a 7 day static preculture of hMSCs encapsulated in alginate scaffolds (labeled Day -7), followed by a 14 day dynamic or static coculture with HUVECs encapsulated in collagen scaffolds (Day 0 - Day 14). B) BMP-2 immunostaining demonstrates increase in BMP-2 production (brown) in hMSCs (dark blue nucleus). C) BMP-2 mRNA expressions in hMSCs significantly increases over 14 days in dynamic culture but stays constant static culture. D) VEGF mRNA expressions in HUVECs significantly increases over 14 days in dynamic culture and shows an increase on day 7 in static before decreasing back close to basal levels. The symbol ‘*’ indicates statistical significance within groups at a timepoint (p<0.05).