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The concentration of glucose, glycerol and lactate in shake‐flask studies. E. coli S17‐1 ΔpflA harbouring two plasmids of pLA' and p3HP2p was cultured in LB medium supplemented with 20 g L−1 glucose, 10 g L−1 glycerol and 2 g L−1 lactate. Blue line, glucose; red line, glycerol; green line, lactate. Error bars represent the standard deviation of experiments conducted in triplicates.
