Table 1.
LA polymerization activities of typical phaC variants
| E. coli | phaC Ps variants | CDM (g L−1) | PHA/CDM (wt%) | LA (mol%) |
|---|---|---|---|---|
| S‐pBLPCAB1 | phaC1 Ps | 4.03 ± 0.27 | 39.22 ± 0.58 | 0 |
| S‐pBLPCAB1‐2 | phaC1 Ps (Q481K S325T) | 4.18 ± 0.13 | 40.12 ± 0.23 | 2.81 ± 0.30 |
| S‐pBLPCAB1‐4 | phaC1 Ps (Q481K S325T E130D S477G) | 3.13 ± 0.31 | 36.94 ± 0.38 | 5.01 ± 1.24 |
| S‐pBLPCAB1‐5 | phaC1 Ps (Q481K S325T E130D S477G F392S) | 3.01 ± 0.22 | 37.51 ± 0.23 | 3.01 ± 0.72 |
| S‐pBLPCAB2 | phaC2 Ps | 3.24 ± 0.09 | 41.50 ± 0.61 | 0 |
| S‐pBLPCAB2‐2 | phaC2 Ps (Q482K S326T) | 4.15 ± 0.43 | 37.71 ± 0.49 | 0 |
| S‐pBLPCAB2‐3 | phaC2 Ps (Q482K S326T S478G) | 3.76 ± 0.52 | 36.13 ± 0.77 | 0 |
Recombinant strains were cultivated for 48 h in shake flasks. The data are the averages of three parallel experiments.
LA, lactate; CDM, cell dry mass.