Table 2.

Culture media used for germination assays of Xeromyces bisporus FRR 0025, Aspergillus penicillioides JH06THJ and Eurotium halophilicum FRR 2471 to characterize germination performance over the entire pH window (see Fig. 3)a

pH of culture mediumb	Stressor type and concentration (M)a	Water activityc
Xeromyces bisporus FRR 0025
2.90	Glycerol (5.5)+sucrose (0.3)	0.720
3.50	Glycerol (5.5)+sucrose (0.3)	0.719
4.60	Glycerol (5.5)+sucrose (0.3)	0.722
5.30	Glycerol (5.5)+sucrose (0.3)	0.721
5.80	Glycerol (5.5)+sucrose (0.3)	0.722
6.40	Glycerol (5.5)+sucrose (0.3)	0.722
7.10	Glycerol (5.5)+sucrose (0.3)	0.722
7.70	Glycerol (5.5)+sucrose (0.3)	0.719
8.10	Glycerol (5.5)+sucrose (0.3)	0.720
8.80	Glycerol (5.5)+sucrose (0.3)	0.718
9.60	Glycerol (5.5)+sucrose (0.3)	0.717
Aspergillus penicillioides JH06THJ
3.00	Glycerol (5.5)+NaCl (1.2)	0.728
3.60	Glycerol (5.5)+NaCl (1.2)	0.725
4.70	Glycerol (5.5)+NaCl (1.2)	0.724
5.50	Glycerol (5.5)+NaCl (1.2)	0.727
6.00	Glycerol (5.5)+NaCl (1.2)	0.726
6.50	Glycerol (5.5)+NaCl (1.2)	0.729
7.00	Glycerol (5.5)+NaCl (1.2)	0.729
7.70	Glycerol (5.5)+NaCl (1.2)	0.724
8.40	Glycerol (5.5)+NaCl (1.2)	0.722
9.00	Glycerol (5.5)+NaCl (1.2)	0.726
9.80	Glycerol (5.5)+NaCl (1.2)	0.723
Eurotium halophilicum FRR 2471
2.80	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.723
3.70	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.721
4.50	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.724
5.50	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.723
6.00	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.725
6.40	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.725
7.00	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.726
7.50	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.724
8.20	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.721
8.90	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.724
9.50	Glycerol (5.5)+NaCl (0.25)+sucrose (0.25)	0.719

^aAll media were based on MYPiA: 1% malt extract, 1% yeast extract, 0.1% KH₂PO₄ and 1.5% (w/v) agar (Williams and Hallsworth, 2009).

^bMedia were buffered by addition of citric acid/Na₂PO₄ (3.00, 3.75, 4.75 and 5.50), PIPES/NaOH (6.0, 6.5 and 7.2) or HEPES/NaOH (7.8, 8.5, 9.2, 10.1) pre‐autoclave. The pH of liquid media was measured using a Mettler Toledo Seven Easy pH‐probe (Mettler Toledo, Greifensee, Switzerland) and for solid media, post‐autoclave using Fisherbrand colour‐fixed pH indicator strips were used (Fisher Scientific, Leicestershire, UK).

^cThe water activity of each medium was measured at the same temperature at which plates were incubated (30°C) and replicate values were within ± 0.001 water activity (see Experimental procedures).