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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2017 Jan 17;104:249–261. doi: 10.1016/j.freeradbiomed.2017.01.028

Figure 5.

Figure 5

Time course of the oxidation of 100 mM se2c5Ura by one equivalent of H2O2 monitored by 77Se-NMR spectroscopy at pH 7.4. After 1.5 h of reaction, one equivalent of Asc was added to the NMR tube and a new 77Se-NMR was acquired (shown by the top spectrum). As is evident, Asc is able to reduce the diselenide form as well as the selenenic acid form back to the original se2c5Ura form.