Table 3.
Putative copper stress related tardigrade transcripts.
| Transcript ID | BLASTX results | BLASTP results | Predicted function | |||
|---|---|---|---|---|---|---|
| E. sigismundi | GB | Hd | Swissprot-ID | Pfam | ||
| Osmoregulatory enzymes | CL1316.Contig3_ Es | Arthropoda [AIM43570.1] | nHd.2.3.1.t06036-RA | P13607|ATNA_DROME | PF00122.18|E1-E2_ATPase | Na/K ATPase subunit alpha |
| (a) 4722; (b) 4983; (c) 52.5341 | (I = 78%; Eval = 0.0; Qc = 62%) | (I = 71%; Eval = 0.0) | (I = 75%; Eval = 0.0) | (Eval = 8.7e-57) | ||
| CL1648.Contig1_ Es | Arthropoda [XP_008556879.1] | nHd.2.3.1.t12553-RA | Q24048|ATPB2_DROME | PF00287.16|Na_K-ATPase | Na/K ATPase subunit beta-2 | |
| (a) 2236; (b) 8266; (c) 184.0348 | (I = 39%; Eval = 4e-58; Qc = 40%) | (I = 40%; Eval = 4e-55) | (I = 37%; Eval = 2e-53) | (Eval = 2.7e-69) | ||
| CL3019.Contig1_ Es | Arthropoda [XP_013777310.1] | nHd.2.3.1.t03974-RA | Q9ULX7|CAH14_HUMAN | PF00194.19|Carb_anhydrase | Carbonic anhydrase | |
| (a) 1329; (b) 4611; (c) 172.7215 | (I = 40%; Eval = 9e-60; Qc = 60%) | (I = 39%; Eval = 1e-41) | (I = 39%; Eval = 9e-46) | (Eval = 6.9e-75) | ||
| Unigene4595_ Es | Arthropoda [JAN40855] | nHd.2.3.1.t13678-RA | Q22460|BCA1_CAEEL | PF00484.17|Pro_CA | Beta carbonic anhydrase | |
| (a) 1137; (b) 1554; (c) 68.0404 | (I = 54%; Eval = 6e-85; Qc = 64%) | (I = 32%; Eval = 4e-31) | (I = 43%; Eval = 4e-51) | (Eval = 4e-51) | ||
| Antioxidant enzymes | Unigene7967_ Es | Arthropoda [XP_013794733.1] | nHd.2.3.1.t08435-RA | Q55GQ5|SODC1_DICDI | PF00080.18|Sod_Cu | Cu-Zn superoxide dismutase |
| (a) 1089; (b) 1546; (c) 70.6737 | (I = 54%; Eval = 5e-49; Qc = 41%) | (I = 49%; Eval = 5e-39) | (I = 52; Eval = 3e-42) | (Eval = 2.0e-44) | ||
| Unigene7176_ Es | Mollusca [AET43974.1] | nHd.2.3.1.t08389-RA | Q8HXP5|SODM_HYLLA | PF02777.16|Sod_Fe_C | Mn Superoxide dismutase | |
| (a) 825; (b) 2116; (c) 127.6845 | (I = 58%; Eval = 9e-93; Qc = 82%) | (I = 59%; Eval = 8e-72) | (I = 60%; Eval = 9e-71) | (Eval = 8.4e-35) | ||
| Unigene9127_ Es | Mollusca [XP_005099721.2] | nHd.2.3.1.t10722-RA | P47791|GSHR_MOUSE | PF07992.12|Pyr_redox_2 | Glutathione reductase | |
| (a) 1619; (b) 3020; (c) 92.8617 | (I = 62%; Eval = 0.0; Qc = 83%) | (I = 56%; Eval = 2e-50) | (I = 59%; Eval = 2e-156) | (Eval = 2e-156) | ||
| CL47.Contig3_ Es | Rotifera [AIL94180.1] | nHd.2.3.1.t11424-RA | Q00277|GPX1_SCHMA | PF00255.17|GSHPx | Gluthathione peroxidase | |
| (a) 1291; (b) 4002; (c) 154.3218 | (I = 58%; Eval = 2e-68; Qc = 42%) | (I = 51%; Eval = 5e-52) | (I = 73%; Eval 2e-18) | (Eval = 4.0e-44) | ||
| Copper transport | Unigene8200_ Es | Annelida [ELT91953.1] | nHd.2.3.1.t02534-RA | Q8K211|COPT1_MOUSE | PF04145.13|Ctr | Ctr copper transporter |
| (a) 975; (b) 1440; (c) 73.5249 | (I = 38%; Eval = 3e-32; Qc = 59%) | (I = 28%; Eval = 5e-10) | (I = 32%; Eval = 1e-20) | (Eval = 2.2e-28) | ||
| Unigene10756_ Es | Mollusca [AEJ08756.1] | nHd.2.3.1.t04635-RA | Q55BB8|COG8_DICDI | PF00403.24|HMA | Copper chaperone Atox1 | |
| (a) 1607; (b) 597; (c) 18.4942 | (I = 51%; Eval = 1e-15; Qc = 12%) | (I = 42%; Eval = 5e-7) | (I 23%; Eval = 2e-8) | (Eval = 1.2e-11) | ||
| Unigene12304_ Es | Arthropoda [XP_015601105.1] | nHd.2.3.1.t07554-RA | Q04656|ATP7A_HUMAN | PF00403.24|HMA | Copper-transporting ATPase | |
| (a) 3397; (b) 3599; (c) 52.7427 | (I = 51%; Eval = 0.0; Qc = 87%) | (I = 49%; Eval = 1e-159) | (I = 49%; Eval = 0.0) | (Eval = 2.2e-14) | ||
E. sigismundi transcripts with information on (a) sequence length (bp), (b) number of raw fragments, (c) FPKM value E. sigismundi transcript sequences are provided in Supplement Table 1. BLASTX best hit results of each E. sigismundi transcript against NCBI (GB), H. dujardini genome (Hd) (http://badger.bio.ed.ac.uk/H_dujardini/), and Swissprot database with the percentage of identity (I), e-value (Eval) and, when provided, query coverage (Qc) within parenthesis. BLASTP best hit results from the Pfam database. Tardigrade transcripts and proposed functions are given in bold.