Table 1.
The relative mRNA levels of genes involved in neurotransmission processes in neuroblastoma IMR-32 cell culture after incubation with GABA, olanzapine, Selank, Selank with GABA, or Selank with olanzapine.
| Gene Symbol | Official full name | GABA | Olanzapine | Selank | Selank + GABA | Selank + Olanzapine | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Fold Regulation | p-value | Fold Regulation | p-value | Fold Regulation | p-value | Fold Regulation | p-value | Fold Regulation | p-value | ||
| ABAT | 4-aminobutyrate aminotransferase | −1.43 | 0.042 | −1.89 | 0.001 | −1.01 | 0.941 | −1.34 | 0.017 | −1.96 | 0.002 |
| ADCY7 | Adenylate cyclase 7 | −2.00 | 0.066 | −2.30 | 0.039 | 1.00 | 0.934 | −1.46 | 0.145 | −2.84 | 0.023 |
| ADORA1 | Adenosine A1 receptor | −1.28 | 0.346 | −1.50 | 0.047 | −1.06 | 0.592 | −1.20 | 0.241 | −1.77 | 0.011 |
| ADORA2A | Adenosine A2a receptor | −3.82 | 0.040 | −2.94 | 0.072 | −1.46 | 0.237 | −2.71 | 0.059 | −3.88 | 0.032 |
| ALDH5A1 | Aldehyde dehydrogenase 5 family, member A1 | −1.20 | 0.251 | −1.51 | 0.008 | −1.02 | 0.761 | −1.15 | 0.136 | −1.65 | 0.005 |
| BCL2 | B-cell CLL/lymphoma 2 | −1.04 | 0.670 | −1.33 | 0.099 | −1.04 | 0.701 | −1.18 | 0.209 | −1.30 | 0.116 |
| BCL2L1 | BCL2-like 1 | −1.75 | 0.050 | −2.22 | 0.005 | −1.10 | 0.362 | −1.25 | 0.096 | −2.62 | 0.002 |
| BDNF | Brain-derived neurotrophic factor | 1.06 | 0.547 | 1.06 | 0.352 | 1.08 | 0.349 | 1.07 | 0.440 | 1.15 | 0.126 |
| BIRC2 | Baculoviral IAP repeat containing 2 | 1.35 | 0.039 | 1.42 | 0.011 | 1.02 | 0.779 | 1.16 | 0.138 | 1.57 | 0.004 |
| CACNA1B | Calcium channel, voltage-dependent, N type, alpha 1B subunit | −1.71 | 0.065 | −2.18 | 0.041 | −1.25 | 0.338 | −1.56 | 0.103 | −2.75 | 0.019 |
| CCND1 | Cyclin D1 | −1.01 | 0.954 | −1.17 | 0.067 | −1.04 | 0.560 | −1.02 | 0.651 | −1.27 | 0.106 |
| CSF2 | Colony stimulating factor 2 (granulocyte-macrophage) | −3.07 | 0.005 | −4.48 | 0.010 | 1.11 | 0.617 | −1.43 | 0.392 | −6.21 | 0.003 |
| CX3CL1 | Chemokine (C-X3-C motif) ligand 1 | −2.31 | 0.004 | −2.70 | 0.026 | 1.06 | 0.712 | −1.29 | 0.286 | −3.74 | 0.004 |
| DRD2 | Dopamine receptor D2 | −1.46 | 0.102 | −1.65 | 0.042 | −1.05 | 0.650 | −1.18 | 0.228 | −1.92 | 0.011 |
| DRD3 | Dopamine receptor D3 | −1.56 | 0.026 | −2.35 | 0.125 | 1.23 | 0.301 | −1.34 | 0.382 | −3.02 | 0.030 |
| DRD4 | Dopamine receptor D4 | −1.70 | 0.115 | −1.69 | 0.139 | −1.19 | 0.489 | −1.30 | 0.323 | −2.15 | 0.056 |
| DRD5 | Dopamine receptor D5 | −1.07 | 0.723 | −1.44 | 0.457 | 1.25 | 0.705 | 1.18 | 0.783 | −1.81 | 0.427 |
| EGR1 | Early growth response 1 | −1.28 | 0.240 | −1.70 | 0.112 | −1.14 | 0.593 | −1.17 | 0.517 | −1.89 | 0.059 |
| FN1 | Fibronectin 1 | −1.59 | 0.009 | −2.06 | 0.001 | 1.01 | 0.792 | −1.28 | 0.029 | −2.23 | 0.0003 |
| FOS | FBJ murine osteosarcoma viral oncogene homolog | −1.76 | 0.084 | −3.06 | 0.001 | −1.21 | 0.293 | −1.29 | 0.061 | −3.63 | 0.002 |
| GABBR1 | Gamma-aminobutyric acid (GABA) B receptor, 1 | −2.11 | 0.121 | −2.38 | 0.097 | −1.01 | 0.820 | −1.34 | 0.320 | −3.11 | 0.044 |
| GABBR2 | Gamma-aminobutyric acid (GABA) B receptor, 2 | −1.13 | 0.534 | −1.11 | 0.620 | 1.07 | 0.771 | 1.06 | 0.745 | −1.25 | 0.357 |
| GABRA1 | Gamma-aminobutyric acid (GABA) A receptor, alpha 1 | −1.36 | 0.106 | −1.54 | 0.021 | −1.14 | 0.318 | −1.13 | 0.340 | −1.35 | 0.086 |
| GABRA2 | Gamma-aminobutyric acid (GABA) A receptor, alpha 2 | 1.33 | 0.076 | 1.35 | 0.022 | −1.06 | 0.477 | 1.24 | 0.039 | 1.33 | 0.039 |
| GABRA3 | Gamma-aminobutyric acid (GABA) A receptor, alpha 3 | 1.16 | 0.083 | 1.09 | 0.436 | 1.03 | 0.782 | 1.11 | 0.243 | −1.00 | 0.981 |
| GABRA5 | Gamma-aminobutyric acid (GABA) A receptor, alpha 5 | 1.60 | 0.026 | 1.62 | 0.009 | 1.05 | 0.641 | 1.25 | 0.059 | 1.86 | 0.005 |
| GABRB1 | Gamma-aminobutyric acid (GABA) A receptor, beta 1 | −1.28 | 0.485 | −1.56 | 0.217 | 1.12 | 0.759 | −1.00 | 0.932 | −1.58 | 0.303 |
| GABRB2 | Gamma-aminobutyric acid (GABA) A receptor, beta 2 | 1.32 | 0.082 | 1.24 | 0.148 | −1.05 | 0.945 | 1.11 | 0.595 | 1.29 | 0.104 |
| GABRB3 | Gamma-aminobutyric acid (GABA) A receptor, beta 3 | 1.32 | 0.001 | 1.09 | 0.060 | 1.04 | 0.191 | 1.07 | 0.011 | 1.12 | 0.002 |
| GABRE | Gamma-aminobutyric acid (GABA) A receptor, epsilon | −1.63 | 0.185 | −1.53 | 0.354 | 1.08 | 0.800 | 1.10 | 0.889 | −1.79 | 0.137 |
| GABRG1 | Gamma-aminobutyric acid (GABA) A receptor, gamma 1 | 1.37 | 0.069 | 1.38 | 0.065 | 1.01 | 0.767 | 1.10 | 0.541 | 1.24 | 0.270 |
| GABRG2 | Gamma-aminobutyric acid (GABA) A receptor, gamma 2 | 1.65 | 0.018 | 1.74 | 0.012 | 1.38 | 0.216 | 1.47 | 0.045 | 1.74 | 0.007 |
| GABRG3 | Gamma-aminobutyric acid (GABA) A receptor, gamma 3 | 1.19 | 0.149 | 1.03 | 0.838 | 1.32 | 0.227 | 1.09 | 0.476 | −1.05 | 0.608 |
| GABRQ | Gamma-aminobutyric acid (GABA) receptor, theta | −1.62 | 0.037 | −1.92 | 0.105 | −1.08 | 0.613 | −1.45 | 0.194 | −2.62 | 0.037 |
| GABRR2 | Gamma-aminobutyric acid (GABA) receptor, rho 2 | −2.03 | 0.063 | −2.47 | 0.043 | 1.01 | 0.905 | −1.50 | 0.204 | −2.78 | 0.037 |
| GAD1 | Glutamate decarboxylase 1 (brain, 67kDa) | −1.07 | 0.622 | −1.36 | 0.468 | 1.24 | 0.785 | 1.15 | 0.860 | −2.06 | 0.249 |
| GLS | Glutaminase | 1.20 | 0.043 | 1.16 | 0.039 | 1.01 | 0.857 | 1.11 | 0.019 | 1.24 | 0.003 |
| GLUL | Glutamate-ammonia ligase | −1.01 | 0.798 | −1.14 | 0.001 | 1.02 | 0.564 | 1.02 | 0.594 | −1.17 | 0.002 |
| GNAI1 | Guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 | 1.22 | 0.064 | 1.23 | 0.062 | 1.05 | 0.587 | 1.18 | 0.067 | 1.34 | 0.008 |
| GNAQ | Guanine nucleotide binding protein (G protein), q polypeptide | 1.57 | 0.039 | 1.69 | 0.014 | 1.07 | 0.504 | 1.24 | 0.055 | 1.87 | 0.001 |
| GPHN | Gephyrin | 1.41 | 0.006 | 1.25 | 0.022 | 1.11 | 0.198 | 1.29 | 0.008 | 1.34 | 0.005 |
| GPR85 | G protein-coupled receptor 85 | −1.15 | 0.218 | −1.43 | 0.015 | −1.24 | 0.073 | −1.15 | 0.056 | −1.58 | 0.004 |
| HCRTR2 | Hypocretin (orexin) receptor 2 | −1.02 | 0.826 | −1.12 | 0.835 | 1.30 | 0.555 | 1.05 | 0.978 | 1.01 | 0.822 |
| HTR1B | 5-hydroxytryptamine (serotonin) receptor 1B | −2.32 | 0.153 | −1.97 | 0.214 | 1.06 | 0.971 | −1.57 | 0.296 | −2.28 | 0.146 |
| HTR2A | 5-hydroxytryptamine (serotonin) receptor 2A | −1.59 | 0.054 | −2.25 | 0.045 | −1.38 | 0.681 | −1.23 | 0.709 | −2.55 | 0.051 |
| HTR3A | 5-hydroxytryptamine (serotonin) receptor 3A | −1.90 | 0.012 | −2.25 | 0.007 | −1.15 | 0.568 | −1.49 | 0.073 | −2.83 | 0.001 |
| HTR3B | 5-hydroxytryptamine (serotonin) receptor 3B | −1.06 | 0.771 | −1.26 | 0.563 | 1.21 | 0.785 | 1.32 | 0.636 | −1.48 | 0.551 |
| ICAM1 | Intercellular adhesion molecule 1 | −1.45 | 0.139 | −2.30 | 0.054 | 1.05 | 0.796 | −1.11 | 0.892 | −2.67 | 0.015 |
| JUN | Jun proto-oncogene | −1.47 | 0.130 | −2.01 | 0.003 | −1.20 | 0.072 | −1.34 | 0.035 | −2.37 | 0.001 |
| JUNB | Jun B proto-oncogene | −3.14 | 0.042 | −5.19 | 0.002 | −1.23 | 0.257 | −1.71 | 0.034 | −6.57 | 0.002 |
| MCHR1 | Melanin-concentrating hormone receptor 1 | −1.27 | 0.148 | −1.58 | 0.011 | 1.05 | 0.689 | −1.37 | 0.004 | −1.84 | 0.001 |
| MMP10 | Matrix metallopeptidase 10 (stromelysin 2) | −2.25 | 0.002 | −2.79 | 0.018 | −1.34 | 0.179 | −1.53 | 0.328 | −3.50 | 0.006 |
| MYC | V-myc myelocytomatosis viral oncogene homolog (avian) | −1.21 | 0.104 | −1.49 | 0.008 | −1.08 | 0.563 | −1.25 | 0.440 | −1.17 | 0.235 |
| NOS2 | Nitric oxide synthase 2, inducible | −1.09 | 0.859 | −1.36 | 0.160 | 1.15 | 0.468 | 1.08 | 0.724 | −1.60 | 0.056 |
| NPFFR1 | Neuropeptide FF receptor 1 | −2.11 | 0.044 | −2.25 | 0.072 | 1.07 | 0.832 | −1.35 | 0.357 | −3.79 | 0.020 |
| NSF | N-ethylmaleimide-sensitive factor | 1.13 | 0.0004 | −1.03 | 0.155 | 1.02 | 0.190 | 1.02 | 0.190 | 1.00 | 0.689 |
| ODC1 | Ornithine decarboxylase 1 | 1.34 | 0.027 | 1.34 | 0.027 | 1.16 | 0.083 | 1.22 | 0.023 | 1.52 | 0.007 |
| P2RX7 | Purinergic receptor P2X, ligand-gated ion channel, 7 | 1.41 | 0.550 | 1.11 | 0.972 | 1.54 | 0.405 | 1.49 | 0.451 | −1.02 | 0.736 |
| PRKCA | Protein kinase C, alpha | −1.29 | 0.015 | −1.57 | 0.0005 | −1.01 | 0.853 | −1.17 | 0.068 | −1.70 | 0.0003 |
| PRKCE | Protein kinase C, epsilon | −1.12 | 0.446 | −1.26 | 0.028 | 1.08 | 0.252 | −1.03 | 0.766 | −1.51 | 0.003 |
| PRLHR | Prolactin releasing hormone receptor | −1.84 | 0.168 | −2.18 | 0.114 | 1.23 | 0.584 | −1.07 | 0.941 | −2.73 | 0.102 |
| SLC1A3 | Solute carrier family 1 (glial high affinity glutamate transporter), member 3 | −1.16 | 0.453 | −1.30 | 0.300 | 1.09 | 0.809 | −1.17 | 0.433 | −1.48 | 0.161 |
| SLC32A1 | Solute carrier family 32 (GABA vesicular transporter), member 1 | −1.73 | 0.050 | −2.32 | 0.075 | 1.02 | 0.838 | −1.33 | 0.416 | −4.01 | 0.010 |
| SLC38A1 | Solute carrier family 38, member 1 | 1.20 | 0.087 | 1.19 | 0.014 | 1.00 | 0.966 | 1.05 | 0.253 | 1.24 | 0.013 |
| SLC6A1 | Solute carrier family 6 (neurotransmitter transporter, GABA), member 1 | −1.42 | 0.302 | −1.84 | 0.134 | 1.19 | 0.573 | 1.00 | 0.888 | −2.30 | 0.114 |
| SLC6A11 | Solute carrier family 6 (neurotransmitter transporter, GABA), member 11 | −1.67 | 0.072 | −1.88 | 0.044 | −1.07 | 0.674 | −1.38 | 0.178 | −2.15 | 0.031 |
| SLC6A13 | Solute carrier family 6 (neurotransmitter transporter, GABA), member 13 | −1.15 | 0.561 | −1.37 | 0.267 | 1.30 | 0.343 | 1.13 | 0.717 | −1.56 | 0.143 |
| SLC8A3 | Solute carrier family 8 (sodium/calcium exchanger), member 3 | −1.20 | 0.379 | −1.52 | 0.095 | −1.10 | 0.514 | −1.11 | 0.514 | −1.83 | 0.039 |
| SNCA | Synuclein, alpha (non A4 component of amyloid precursor) | 1.47 | 0.113 | 1.71 | 0.026 | −1.09 | 0.624 | 1.17 | 0.358 | 1.84 | 0.013 |
The data in bold denote changes in the expression by 1.5 times or more, with a significance level (p < 0.05). The red color denotes the increase in expression of genes; the blue color denotes the decrease in expression of genes.