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Harvested filaments from of CS29544, gene knockout, and complementation strains. Gene knockouts: ΔflhA, ΔfliG, ΔmotAB, ΔfliC, ΔflaA, ΔflaAΔfliC, and FliC complements: ΔfliC/cfliC and ΔflaAΔfliC/cfliC. Each lane was loaded with ~2 μg of total protein. FliC expected size 28.9 kDa.
