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. 2017 Feb 28;8:301. doi: 10.3389/fmicb.2017.00301

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Copyright © 2017 Hoeflinger and Miller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Autoaggregation, motility, and flagellation of FliC complementation strains. (A) Time course of autoaggregation in BHI broth over 6 h. FliC complements: ΔfliC/cfliC and ΔflaAΔfliC/cfliC. Experiment is mean ± standard error of three independent replicates. (B) Autoaggregation tube assays in BHI broth after static incubation for 6 h. (C) Motility agar plates centrally inoculated and imaged after 24 h, flagellation as detected by crystal violet staining at 1,000 × total magnification and TEM, bar = 4 μm.