Table 1.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics or purpose | Internal number | Source or references |
|---|---|---|---|
| C. SAKAZAKII STRAINS | |||
| CS29544 | Cronobacter sakazakii ATCC 29544, wild-type | MJM187 | ATCC |
| CS29544 pKD46 | CS29544 harboring pKD46 | MJM396 | This study |
| flhA_CV (2.10) | CS29544 flhA nonautoaggregating variant | MJM387 | This study |
| fliG_CV (3.6) | CS29544 fliG nonautoaggregating variant | MJM388 | This study |
| ΔflhA | CS29544 flhA | MJM425 | This study |
| ΔfliG | CS29544 fliG | MJM426 | This study |
| ΔmotAB | CS29544 motAB | MJM476 | This study |
| ΔfliC | CS29544 fliC | MJM477 | This study |
| ΔflaA | CS29544 flaA | MJM478 | This study |
| ΔflaA pKD46 | CS29544 flaA harboring pKD46 | MJM480 | This study |
| ΔflaAΔfliC | CS29544 flaAfliC | MJM484 | This study |
| ΔfliC/cfliC | CS29544 fliC/fliC complementation vector | MJM481 | This study |
| ΔflaAΔfliC/cfliC | CS29544 flaAfliC fliC complementation vector | MJM483 | This study |
| E. COLI STRAINS | |||
| DH5α | pKD3 and pCP20 host strain | MJM363, MJM364 | Gift from J. M. Slauch |
| MG1655 | pKD46 host strain | MJM365 | Gift from J. M. Slauch |
| Top10 | cfliC host strain | MJM423 | Invitrogen |
| PLASMIDS | |||
| pCP20 | bla cat cI857 λPRflp | Cherepanov and Wackernagel, 1995 | |
| pKD3 | bla FRT cat FRT | Datsenko and Wanner, 2000 | |
| pKD46 | bla PBAD gam bet exo | Datsenko and Wanner, 2000 | |
| cfliC | 1 kb fragment fliC native promoter and ORF cloned into pET-11a | pMJM49 | This study |