Table 2.
Primer sequences used to create and confirm gene knockouts.
| Locus | Primers | Sequence (5′–3′) |
|---|---|---|
| cat | c2_F | GATCTTCCGTCACAGGTAGG |
| c1_R | TTATACGCAAGGCGACAAGG | |
| flhA | flhA_ko_F | ATCACCAAGGGTGCGGGGCGTATCGCGGAAGTGGGCGCGC |
| flhA_ko_R | GATAGGCTTCACCGCTGCCGATTTCCACGCCTTTCATCAG | |
| flhA_flank_F | GAAATCGGTCAGCAGATCC | |
| flhA_flank_R | CAGACCACGACCGTCATCAC | |
| fliG | fliG_ko_F | TGACCATCGGTGAAGACCGCGCGGCGGAGGTGTTCAAACA |
| fliG_ko_R | ATAGCTTTCTGTTCGTTTTCCACCTGAGACAGACGCACCG | |
| fliG_flank_F | AGCGTAAAGAAGTGGAAGAG | |
| fliG_flank_R | CGTCGACTTCTTCGCTTTC | |
| motAB | motAB_ko_F | TGAACATCCTCGCCATAGCCAACAGCGGAAGGATGATGTC |
| motAB_ko_F | AAATGTCTGATAAAAATCGCTAATATCCATACTCACGCTA | |
| motAB_flank_F | CGTAAACTTTCGCGAGATGCTG | |
| motAB_flank_R | CGCGGAATATGGCATTTAGCTG | |
| fliC | fliC_ko_F | CAACCTGAACAAATCTCAGTCTGCTCTGGGCACTGCTATC |
| fliC_ko_F | GAAAGCTTGAGCGTCTATTAACGCAGCAGGGACAGCATGG | |
| fliC_flank_F | ATGCAGACGCAGGCTATTGAG | |
| fliC_flank_R | TCCGGCTATATCTGTCGCAAC | |
| flaA | flaA_ko_F | GCCACGCCGGTTGAGTCAGCGTCCGGGCAGGCGCGTGACG |
| flaA_ko_F | GCCTTGTTAACGACTCTCCGATGTGCGAAACGGCAACCCT | |
| flaA_flank_F | CTTCCCGCTGACCATTTC | |
| flaA_flank_R | AACGTGCCGTCCATATCC |