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The effect of -induced or -inhibited autophagy on PHEV replication. (A,B) Neuro-2a cells were infected with PHEV in the presence or absence of rapamycin (100 nM) or 3-MA (2 mM). At 24 h and 48 hpi, the cells were harvested and analyzed by immunoblotting using anti-LC3, anti-PHEV, and anti-β-actin antibodies.
