Figure 2.

Neuronal labeling in the CA1 region of the hippocampus by SR101 does not require OATP1C1. (A) Labeling of slices from Oatp1c1 knock out mice (Mayerl et al., 2012) using 1 μM SR101 for 20 min at 34°C followed 10 min of de-staining in aCSF (Schnell et al., 2012). (B) Same protocol except that 100 μM of carbenoxolone (CBX) was added during the staining procedure. Note that labeling of neurons was not reduced by CBX. In (C,D) the staining procedure was altered and 165 μM SR101 was applied at room temperature (Kantor et al., 2012). This protocol leads to a much brighter staining of neurons (see lookup table that was used for all four panels) but not to a staining of astrocyte-like cells. Again application of 100 μM CBX did not block the neuronal labeling. (E) Statistical analysis of the fluorescence intensity of neurons. Threshold based pixel analysis using ImageJ software. The asterisks indicated significance between 165 μM and 1 μM SR101 treatments. ANOVA with all pairwise multiple comparison procedures (Holm-Sidak method; p < 0.05; n = 3 mice) using SigmaPlot software.