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. 2017 Feb 28;11:44. doi: 10.3389/fncel.2017.00044

Figure 3.

Figure 3

Effects of neurosteroids on SR101 uptake in the hippocampus. (A) Astroglial EGFP-fluorescence (green) and SR101-fluorescence (red) in the hippocampus after a staining in control conditions (1 μM SR101 for 20 min at 34°C). (B) Reduced SR101-labeling of hippocampal astrocytes when the neurosteroid allopregnanolone sulfate (APAS, 100 μM) was included in the staining solution. Both figures show maximum intensity projection 51 stacks (2 μm distance). (C–F) Images from single layer for EGFP (C,D) and SR101 (E,F). Note the reduction of the intensity of SR101 after application of APAS (F). (G–I) Statistical analysis: (G) The reduction of the SR101-intensity by 100 μM APAS is significant (Mann-Whitney Rank Sum Test; p = 0.032; n = 5 slices; 3 mice). (H) Additionally the number of SR101 positive cells than can be identified after APAS is reduced (t-Test; p = 0.040). (I) The APAS-induced reduction of SR101 intensity in EGFP-positive cells and EGFP-negative SR101 labeled cells is not different (Mann-Whitney Rank Sum Test; p = 0.548). Asterisks in (G,H) indicate significance (p < 0.05).