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. 2017 Feb 28;8:238. doi: 10.3389/fpls.2017.00238

Figure 3.

Figure 3

AR156-mediated ISR through a JA/ET signaling pathway and NPR1. Plants pretreatment, B. cinerea infection and disease symptom observation are performed as in figure 1. (A) Disease symptoms in wild type (Col-0), SA (sid2-2, NahG, and npr1) and JA/ET (jar1, ein2) signaling pathway mutant plants (2 dpi). (B) Leaf necrosis development was evaluated at 2 dpi by determining the average necrosis diameter on three leaves per plants for six plants. (C) In planta growth of B. cinerea in the Col-0, sid2-2, NahG, jar1, ein2 and npr1 mutant plants. Measurement of fungal growth was carried out by simultaneous quantification of the transcript levels of B. cinerea Actin gene (BcActin) and the Arabidopsis Actin gene (AtActin). Relative fungal growth was determined by ratios of BcActin/AtActin. A Student's t-test was used to determine significant differences between the AR156-treated sample and the control (**P < 0.01). The means values ± SD (n = 12) from one representative experiment among three independent repeats are shown.