FIGURE 2.

The effect of DCPIB and FFA with and without zinc on human vacuolar pH (A) and area (B). The acidic vacuole population was removed as described in the methods section; the vacuoles excluded are those with a SNARF Fluorescence ratio (SFR) below 1.36. Each point represents an individual measurement (see Table 1 for the exact number of measurements and statistics); each condition has an overlaying boxplot showing the median and interquartile range. DCPIB (20 μM), FFA (100 μM), Zn (Zn²⁺ 100 μM), D+Zn (DCPIB with Zn²⁺), F+Zn (FFA with Zn²⁺). Differences from control neutrophils: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Differences from neutrophils incubated with Zn2+: †p < 0.05, ††p < 0.01, †††p < 0.001.