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Vacuolar pH (A) and area (B) measured in the single knockout KCC3-/- mice, and in the double knockout HVCN1-/-/KCC3-/- mice. Each square represents an individual measurement with an overlaying boxplot showing the median and interquartile range. A small difference was found between the vacuolar pH of WT and KCC3-/- neutrophils which was statistically significant (∗∗∗p < 0.001). The SFR cut-off value for the HVCN1-/- and double knockout mice was 1.31.
