Table 4.
A compilation of the averaged results of nine online datasets of mRNA expression in neutrophils.
| Gene symbol | Gene description | Relative expression (Human) | Human | Mouse |
|---|---|---|---|---|
| Oxidase components | ||||
| NCF2 | Neutrophil cytosolic factor 2 | 98.50 | ||
| CYBA | Cytochrome b-245, alpha polypeptide | 86.79 | ||
| NCF4 | Neutrophil cytosolic factor 4, 40 kDa | 94.99 | ||
| CYBB | Cytochrome b-245, beta polypeptide | 61.05 | ||
| HVCN1 | Hydrogen voltage-gated channel 1 | 78.57 | ||
| Chloride channels | ||||
| CLIC1 | Chloride intracellular channel 1 | 95.92 | Averaimo et al., 2010 | ∗ |
| BEST1 | Bestrophin 1 | 59.91 | ∗Fischmeister and Hartzell, 2005 | |
| CIC7 | Chloride channel, voltage-sensitive 7 | 41.53 | ∗Graves et al., 2008 | ∗ |
| ClC3 | Chloride channel, voltage-sensitive 3 | 35.36 | Moreland et al., 2006 | ∗Moreland et al., 2006 |
| Potassium channels | ||||
| KCNJ15 | Potassium inwardly-rectifying channel, subfamily J, member 15 | 93.69 | ∗ | |
| KCNE3 | Potassium voltage-gated channel, Isk-related family, member 3 | 77.44 | Barro-Soria et al., 2015 | ∗ |
| KCNJ2 | Potassium inwardly-rectifying channel, subfamily J, member | 80.66 | ∗Vicente et al., 2003 | Masia et al., 2015 |
| KCNQ1 | Potassium voltage-gated channel, KQT-like subfamily, member 1 | 48.37 | ∗ | |
| KCNAB2 | Potassium voltage-gated channel, shaker-related subfamily, beta member 2 | 43.76 | ||
| KCNK7 | Potassium channel, subfamily K, member 7 | 41.97 | ||
| KCNH7 | Potassium voltage-gated channel, subfamily H (eag-related), member 7 | 32.57 | ||
| KCNH3 | Potassium voltage-gated channel, subfamily H (eag-related), member 3 | 7.02 | ||
| Transient receptor potential channels | ||||
| TRPM6 | Transient receptor potential cation channel, subfamily M, member 6 | 31.06 | ||
| MCOLN1 | Mucolipin 1 | 31.60 | ∗Bach et al., 1999 | |
| TRPV2 | Transient receptor potential cation channel, subfamily V, member 2 | 17.94 | ∗Link et al., 2010 | |
| TRPV6 | Transient receptor potential cation channel, subfamily V, member 6 | 11.15 | Heiner et al., 2003b | |
| Solute carriers | ||||
| SLC12A6 (KCC3) | Solute carrier family 12 (potassium/chloride transporters), member 6 | 92.47 | Sun et al., 2012 | ∗Sun et al., 2012 |
| SLC26A6 | Solute carrier family 26, member 6 | 29.45 | ∗Ishiguro et al., 2006 | |
| SLC12A4 (KCC1) | Solute carrier family 12 (potassium/chloride transporters), member 4 | 14.34 | ||
Genes were ranked on relative mean expression levels; a more detailed explanation for acquiring the data is described in the methods. Expression levels of components of the neutrophil NOX2 oxidase system, gp91phox, p22phox, NCF1, NCF2, and HVCN1 are shown for comparison. The table has been edited to show channels linked to neutrophils, NOX systems or other immune cells in the literature. Asterisks denote which genetic models we have tested. Shaded rows indicate genetic models not yet investigated. The source of each data set can be found in Supplementary Table S5, while the full list of gene expression can be viewed in Supplementary Table S6. We also tested CFTR, ClC4, TRPA1, TRPC1, TRPC3, TRPC5, TRPC6, TRPM2, and TRPV1 for which expression levels were very low or non-existent.