Table 5.
Vacuolar pH (A) and area (B) experimental measurements and statistics for the knockout mouse models presented in Figures 5–8.
| (A) Vacuolar SNARF ratio | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| N | Control | No. of points | Control median (SFR/pH) | Knockout | No. of points | Knockout median (SFR/pH) | p-value | ||
| 3 | Wt | 1269 | 0.9 | 7.5 | ClC3-/- | 1116 | 0.9 | 7.5 | 0.268 |
| 2 | HVCN1-/- | 1130 | 3.6 | 11.1 | HVCN1-/-/ClC3-/- | 1157 | 3.7 | 11.2 | 0.020 |
| 4 | Wt | 1637 | 0.9 | 7.4 | KCC3-/- | 1340 | 0.9 | 7.5 | 2.0 × 10-4 |
| 3 | HVCN1-/- | 383 | 2.8 | 9.8 | HVCN1-/-/KCC3-/- | 472 | 2.8 | 9.9 | 0.165 |
| 3 | Wt | 589 | 1.2 | 8.0 | CLIC1-/- | 780 | 1.2 | 8.0 | 0.598 |
| 3 | HVCN1-/- | 1594 | 3.5 | 10.8 | HVCN1-/-/CLIC1-/- | 1596 | 3.5 | 10.8 | 0.174 |
| 3 | Wt | 371 | 1.0 | 7.8 | TRPM2-/- | 399 | 1.0 | 7.8 | 0.087 |
| 1 | HVCN1-/- | 118 | 1.9 | 9.0 | HVCN1-/-/TRPM2-/- | 82 | 1.8 | 8.9 | 0.120 |
| 3 | Wt | 898 | 1.0 | 7.8 | KCNJ15-/- | 727 | 1.1 | 7.9 | 0.016 |
| (B) Vacuolar area | |||||||||
| N | Control | No. of points | Control median (±SD) | Knockout | No. of points | Knockout median (±SD) | p-value | ||
| 3 | Wt | 281 | 10.8 (4.9) | ClC3-/- | 273 | 11.2 (4.5) | 0.650 | ||
| 2 | HVCN1-/- | 549 | 13.4 (6.7) | HVCN1-/-/ClC3-/- | 570 | 14.2 (6.1) | 0.109 | ||
| 3 | Wt | 445 | 11.2 (3.4) | KCC3-/- | 458 | 11.3 (3.9) | 0.469 | ||
| 3 | HVCN1-/- | 389 | 16.7 (6.4) | HVCN1-/-/KCC3-/- | 287 | 17.3 (5.5) | 0.554 | ||
| 3 | Wt | 266 | 12.9 (4.2) | CLIC1-/- | 346 | 13.2 (4.5) | 0.167 | ||
| 3 | HVCN1-/- | 696 | 15.1 (7.4) | HVCN1-/-/CLIC1-/- | 650 | 12.8 (6.8) | 6.7x10-7 | ||
| 3 | Wt | 162 | 11.7 (5.3) | TRPM2-/- | 208 | 11.0 (5.1) | 0.569 | ||
| 1 | HVCN1-/- | 78 | 17.1 (9.9) | HVCN1-/-/TRPM2-/- | 39 | 18.7 (11.4) | 0.830 | ||
| 3 | Wt | 633 | 9.9 (3.9) | KCNJ15-/- | 387 | 10.4 (4.6) | 0.003 | ||
N = number of experiments performed, no. of points describes the total number of individual measurements. The control and corresponding knockout medians are accompanied with the SFR and converted vacuolar pH and standard deviation (SD) for the vacuolar area.