FIGURE 2.

SDH2.3 expression is reduced in bzip53 and ABA deficient mutant seeds. SDH2.3 transcript levels were determined by qRT-PCR and normalized to clathrin mRNA levels for each sample. (A) Expression in dry seeds. Values represent means ± SD of three biological replicates (two technical replicates each). Two asterisks indicate a value for bzip53 seeds that was determined by the t-test to be significantly different from the other three genotypes (p < 0.01). (B) Expression in aba mutant seeds. The last two steps of the ABA biosynthetic pathway are impaired in aba2 and aba3 mutants, respectively. Values represent means ± SD of three biological replicates (two technical replicates each). Three asterisks indicate a value in Col-0 seeds that was determined by the t-test to be significantly different from the four aba mutants at p < 0.001.