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. 2017 Feb 28;8:277. doi: 10.3389/fpls.2017.00277

FIGURE 3.

FIGURE 3

bZIP53 and bZIP10 transcription factors activate the SDH2.3 promoter. (A) Arabidopsis protoplasts were transfected with a reporter construct (SDH2.3 promoter fused to GUS) and effector constructs for expression of either bZIP or ABI3 transcription factors under the control of the CaMV35S promoter. A control plasmid expressing NAN was included in all transfections to normalize GUS activities for differences in transfection efficiencies. x-axis values are given as GUS activity to NAN activity (arbitrary units). NE is no effector(s) (replaced by the pUCpSS vector). Values are means ± SD of three replicates. Asterisks indicate values that were determined by the t-test to be significantly different from that in NE (∗∗p < 0.01; ∗∗∗p < 0.001). All other GUS/NAN values are not significantly different form that in NE. (B) Arabidopsis protoplasts were transfected with either the same reporter as in (A) (wild type SDH2.3 promoter fused to GUS) or a reporter construct with a mutated version of the SDH2.3 promoter, in which a modified ABRE2 element was fused to GUS. Effector constructs expressed bZIP transcription factors. Values are means ± SD of three replicates. Promoter activities in the presence of bZIP53+bZIP10 are significantly different from activities in NE (p < 0.001, asterisks not shown). Two asterisks indicate a significant difference at p < 0.01.