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Flow cytometric gating of PBMC subsets for determination of ZMIZ1 and RPS6 protein expression. (A) ZMIZ1 panel: progressive gating of subsets of interest, and ZMIZ1 median fluorescence intensity of DC and monocyte subsets relative to isotype control; (B) RPS6 panel: gating of live PBMCs and their median fluorescence intensity of RPS6 relative to isotype control.
