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. 2017 Feb 28;8:312. doi: 10.3389/fmicb.2017.00312

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Copyright © 2017 Aloni-Grinstein, Schuster, Yitzhaki, Aftalion, Maoz, Steinberger-Levy and Ber.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Incubation time required to reach O.D._{630 nm} = 0.035 following SST-IMS of Y. pestis blood cultures. Y. pestis EV76 blood cultures at various initial concentrations were subjected to the SST-IMS procedure. Aliquots of 100 μl of the SST-IMS outputs from each blood culture were plated in 6 replicates in microtiter plates. The microtiter plates were incubated in a plate reader for 48 h at 28°C in ambient air, and the absorbance at O.D._{630 nm} was read at 1-h intervals until reaching 0.035. Linear regression of log concentration versus time yielded the following prediction equation: Time (h) = -6.1926x + 52.311.