Table 1.
Primers and linkers used in this study and the corresponding virus region amplified
| Primer name | Sequence (5′-3′) | Amplified region |
|---|---|---|
| NepoWP-5UTR-Rin1* | GGTAAAAAAACAAATTAAGACTATTATTATATTTGC | 0 - 261 |
| NepoWP1_Fn | TATGGATAGCGCCATAGTTTTACCTTAT | 197 - 486 |
| NepoWP1_Rn | CTGTATAGGCAGAAATGGTGGTTATACC | |
| Nepo1-WP_Fa | TCTCCCACTTATATCCAAGGTTCTTCC | 402 - 1928 |
| Nepo1-WP_Ra | CCGCCACTTTTTGCTGCTCTG | |
| NepoWP-RdRp-F1 | CAGCCACCAAGACGGAACCAGCC | 2671 - 4648 |
| NepoWP-RdRp-R1 | GGTTCTTTAATTGGTACATTGAC | |
| NepoWP-F2 | CAGATTATATGCTCTGAGTGGG | 5384 - 6340 |
| NepoWP-R2 | GGCAAGCCACCGACACCAGCTCC | |
| NepoWP-RdRp-F3 | GCAAAGCGTGTCACAGGTATACG | 6184 - 7082 |
| NepoWP-3UTR-R3 | GGACAAACTTATCTCATCGCCAC | |
| Nepo1-WP_3UTR-F* | CATTCAGGTCGAAGTTTACAGTTGCC | 6797 - 7154 |
| Nepo2-WP_5UTR-R* | CTACCAAGGGCTTCAGCTTTGGC | 0 - 285 |
| Nepo2-WP_3UTR-F* | GACGGCCGGACCTTTCTCTCC | 4204 - 4527 |
| Modban linker | A5’pp CTG TAG GCA CCA TCA AT/ddC/ | |
| Bantwo linker | ATC GTrA rGrGrC rArCrC rUrGrA rArA |
* Used together with RACE primer