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. 2017 Jan 3;21(2):393–398. doi: 10.1007/s00792-016-0912-0

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Morphological integrity of Halobacterium salinarum in NaCl solutions. Cells were grown in 4.3 M NaCl and 81 mM MgSO₄ and dialyzed against a solution of 81 mM MgSO₄. a Plot of the relative amount of intact, lysed, and semi-lysed cells with respect to the remaining NaCl concentration after dialysis. Analysis of 60 microscopic frames containing 8139 cells in total. Curve fits were calculated using Igor Pro 6.3 (WaveMetrics®). b Selected cells illustrating intact, lysed, and semi-lysed states (from top to bottom) in the light microscope (DIC imaging)