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. 2017 Jan 3;21(2):393–398. doi: 10.1007/s00792-016-0912-0

Fig. 2.

Fig. 2

Cryo-electron microscopy of intact Halobacterium salinarum cells after vitrification in 3 M NaCl plus 81 mM MgSO4. Cells were grown for 72 h (OD600 0.9). Bright cell inclusions are gas vesicles. The single small gas vesicle in the “empty” carbon hole (top right) of the grid originates from a lysed cell. The low signal-to-noise ratio of the image is due to the limited electron dose (≈60 e2), the thickness of the ice layer (>500 nm) and the contrast contribution of the salt