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. 2017 Feb 21;18(8):2007–2017. doi: 10.1016/j.celrep.2017.01.079

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© 2017 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Frataxin Precursor Interacts with RNF126

HEK293 cells were transiently transfected with the indicated constructs. Protein extracts were collected 40 hr post-transfection.

(A) Proteins were immunoprecipitated with anti-frataxin (anti-fxn) antibody. Arrow indicates RNF126 coprecipitated with frataxin. ^∗Antibody heavy chain.

(B) Proteins were immunoprecipitated with anti-RNF126 antibody. Upper left panel: short exposure. Upper right panel: long exposure. ^∗Non-specific band.

Immunoprecipitate (IP) and total cell extract (tot) were probed on a WB with anti-frataxin (anti-fxn) antibody or anti-RNF126 antibody, as indicated. Pre, precursor int, intermediate; mat, mature frataxin; Ub-fxn, ubiquitinated frataxin; ex RNF126, overexpressed exogenous RNF126; end RNF126, endogenous RNF126.