Figure 6.

Functional effects of Intrepicalcin on single RyR1 acitvity. RyR1 single channel was incorporated by inducing fusion of skeletal muscle heavy SR vesicles into lipid bilayers. The channel activities were recorded and analyzed as described in Methods. RyR1 single channel in the absence of Intrepicalcin was used as control. Intrepicalcin was added into the cis chamber. The arrow marking ‘c’ shows the zero current level when the channel is in the fully closed state, and the arrow marking ‘s’ shows the Intrepicalcin-stabilized subconductance state of the channel. Current histograms from 10,000–30,000-ms segments of channel activity are shown at the right side of the traces for control or 100 nM Intrepicalcin. Experiments were tested at least three times.