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. 2017 Feb 28;108(2):267–276. doi: 10.1111/cas.13128

Figure 2.

Figure 2

Sialyltransferase ST3GAL6 regulated invasion, migration, and proliferation of hepatocellular carcinoma (HCC) cells. (a–f) MHCC97‐L cells were transfected with ST3GAL6 or control vector; MHCC97‐H cells were transfected with siST3GAL6 or scrambled siRNA (siSCR). (a) Relative expression of ST3GAL6 was detected by quantitative real‐time PCR and Western blot analysis in the two hepatoma cell lines after transfection. Proliferation curves according to the results of CCK‐8 assay (b) and representative results of the colony formation assay (c) for MHCC97‐L and MHCC97‐H cells with different transfections. Representative results of the Transwell invasion assay (d) and wounding healing assay (e, f) for MHCC97‐L transfected with ST3GAL6 (or control vector) and MHCC97‐H cells transfected with siST3GAL6 (or siSCR). Each experiment was independently repeated at least three times. Data are presented as the mean ± SD. *P < 0.05 compared with the control.