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. 2017 Feb 28;108(2):267–276. doi: 10.1111/cas.13128

Figure 3.

Figure 3

MicroRNA‐26a (miR‐26a) was a negative regulator of sialyltransferase ST3GAL6. (a) Relative expression of 20 types of predicted miRNAs in MHCC97‐H hepatocellular carcinoma (HCC) cells compared with MHCC97‐L cells. (b) Relative expression level of miR‐26a in HCC tissues and adjacent non‐tumor tissues (28 pairs). (c) Correlation of miR‐26a to ST3GAL6 mRNA levels in 28 pairs of HCC tissues using Spearman's correlation analysis. (d) Wild‐type (WT) or mutant (MUT) miR‐26a target sequences of the ST3GAL6 mRNA 3′‐UTR. (e) Relative luciferase activity of HEK 293T cells after cotransfection with WT or MUT ST3GAL6 3′‐UTR reporter genes or pGL3‐control vector and miR‐26a mimics or miR‐control. (f) Western blot analysis of ST3GAL6 protein expression in MHCC97‐H and MHCC97‐L cells. (g) Relative expression of ST3GAL6, detected by quantitative real‐time PCR after transfection in MHCC97‐H and MHCC97‐L cells. Each experiment was independently performed at least three times. Data are presented as mean ± SD. *P < 0.05.