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. 2017 Feb 28;108(2):267–276. doi: 10.1111/cas.13128

Figure 4.

Figure 4

MicroRNA‐26a (miR‐26a) suppressed the proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells by negatively regulating sialyltransferase ST3GAL6. (a–f) MHCC97‐H cells were cotransfected with target mRNA (ST3GAL6 or control vector) and miRNA mimics (miR‐26a or miR‐NC); MHCC97‐L cells were cotransfected with siRNA (siST3GAL6 or scrambled siRNA [siSCR]) and miRNA inhibitor (anti‐miR‐26a or anti‐miR‐NC). (a) Western blot analysis of ST3GAL6 protein expression in MHCC97‐H and MHCC97‐L cells. (b–f) Upregulation of ST3GAL6 reversed the inhibitory effect of miR‐26a in MHCC97‐H cells and knockdown of ST3GAL6 abrogated the promoting effect of anti‐miR‐26a in MHCC97‐L cells. Proliferation curves according to results of the CCK‐8 assay in MHCC97‐H (b) and MHCC97‐L (c). Representative results of the colony formation assay in MHCC97‐H (d) and MHCC97‐L (e) cells. Representative results of wound‐healing assay in MHCC97‐H (h) and MHCC97‐L (i) cells and Transwell invasion assay in MHCC97‐H (f) and MHCC97‐L (g) cells. Each experiment was independently repeated at least three times. Data are presented as mean ± SD. *P < 0.05. OD, optical density.