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. 2017 Feb 28;108(2):267–276. doi: 10.1111/cas.13128

Figure 5.

Figure 5

Sialyltransferase ST3GAL6 mediated the regulation of microRNA‐26a (miR‐26a) to the protein kinase B/mammalian target of rapamycin (Akt/mTOR) signaling pathway. (a) In MHCC97‐H hepatocellular carcinoma cells with miR‐26a overexpression, the expression levels of ST3GAL6, phosphorylated (p‐)Akt, and p‐mTOR were significantly decreased compared with the control. ST3GAL6 treatment abrogated the decreased expression of these genes induced by miR‐26a. (b) Knockdown of ST3GAL6 by siST3GAL6 inhibited expression of p‐Akt and p‐mTOR, similar to miR‐26a. (c) In MHCC97‐L cells with miR‐26a downregulation, the expression levels of ST3GAL6, p‐Akt, and p‐mTOR were dramatically increased compared with the control. siST3GAL6 treatment reversed the promoting effect of decreased miR‐26a expression. (d) Overexpression of ST3GAL6 promoted the expression of p‐Akt and p‐mTOR, similar to anti‐miR‐26a. Each experiment was independently repeated at least three times. Data are presented as mean ± SD. *P < 0.05.