Figure 7.

Mapping of monoclonal antibodies through competitive ELISA. Inhibition of biotinylated mouse anti-ferret Ig mAb binding to purified ferret Ig was performed to identify overlapping epitope recognition. Competitive inhibition of (a) 4-B10, (b) 4-E3, (c) 8-H9, (d) 11-E3, and (e) 6-B7 were performed using the indicated concentration of biotinylated mAb. The x-axis indicates concentration of unlabeled mouse anti-ferret Ig mAb competitor, and the homologous unlabeled mAb are depicted in red. Percent of maximal binding by the respective biotinylated mAb is plotted ± SD of duplicates. Data in (a)–(e) are representative of two independent experiments.