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. 2017 Feb 26;8(1):86–94. doi: 10.4331/wjbc.v8.i1.86

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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.

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Protection against menadione-induced mitochondrial depolarization with L-carnitine. C2C12 cells were either untreated (white histogram) or pretreated with 500 μmol/L of L-carnitine (dark histogram) and incubated for 24 h with desired concentration of menadione (from 0 to 12 μmol/L). Mitochondria integrity was evaluated using DiOC6(3) probe staining; fluorescent cells were counted using a cytometer. Results are presented as the mean value ± SD. Two different values (P < 0.005) are indicated by a star above the two histograms.