Table 1.
Studies that have evaluated the association between age and human ADSC and adipocyte functionality (ordered by sample size)
| Reference | LOE | Sample size (n) | Subjects | F (n or %) | Age (years; mean ± SD or only mean) | Age (years) (subset categories) | Outcome: ADSC yield | Outcome: differentiation potential |
|---|---|---|---|---|---|---|---|---|
| van Harmelan et al. 2003 [34] | 2 | 189 | Elective breast reduction procedure | 100% | 16–73 | - | No significant correlation between age and ADSCs per gram of tissue | No significant correlation between age and ADSC differentiation |
| Faustini et al. 2010 [29] | 2 | 125 | Donor sites varied by gender. Men had more resected samples; women had more lipoaspirates | 70% | 15–87 (51.31) | - | No significant correlation between age and ADSC yield (stratified by gender) | - |
| Yu et al. 2010 [36] | 2 | 64 | Elective liposuction surgery or abdominoplasty | 90.6% | 18–66 (43.6 ± 11.1) | - | No significant correlation between age and ADSC yield | - |
| Madonna et al. 2011 [42] | 2 | 52 | Patients undergoing abdominal surgery with varying cardiovascular history | 38% | 68 ± 13 | 40–54 (12) 55–65 (18) 66–92 (22) |
Significant negative correlation between age and ADSC yield (subset matched for co-morbidities) | |
| Harris et al. 2010 [30] | 2 | 50 | Lipoaspirate from abdomen in patients undergoing elective vascular procedures Multiple co-morbidities | 36% | 59 ± 16 | <70 (35) >70 (15) |
No significant correlation between age and ADSC yield | No significant correlation between ADSC differentiation and age |
| Alt et al. 2012 [38] | 2 | 40 | Adipose tissue from healthy donor tissue | - | 15–71 | <20 (15) 30–40 (17) > 50 (8) |
Significant negative correlation between age and ADSC yield | Significant negative correlation between age and ADSC proliferation and differentiation rate |
| Mojallal et al. 2011 [31] | 2 | 42 | Elective liposuction—abdomen | 100% | 27–71 | - | No significant correlation between age and ADSC yield and proliferation | - |
| Choudhery et al. 2014 [37] | 2 | 29 | Lipoaspirate from healthy men and women | 69% | 24–67 | <30 (8) 35–50 (10) > 60 (11) |
Significant negative correlation between donor age and ADSC yield | Significant negative correlation between age and ADSC proliferation rate Adipogenic differentiation was independent of age Osteogenic and chondrogenic potential decreased with age |
| Hauner et al. 1989 [121] | 2 | 27 | Subcutaneous tissue from patients undergoing elective abdominal surgery | 37% | 20–83 | - | Significant negative correlation between age and ADSC yield (in both genders) | Significant negative correlation between age and ADSC proliferation (in both genders) |
| de Girolamo et al. 2009 [122] | 2 | 26 | Lipoaspirate from healthy women | 100% | 21–68 | <35 (12) > 45 (14) |
Significant positive correlation between age and ADSC yield | Proliferation rates higher in younger women but not statistically significant No significant impact of age on adipogenic differentiation potential Significant negative correlation of age with osteogenic potential |
| Padoin et al. 2008 [32] | 2 | 25 | Elective liposuction—multiple donor sites | 100% | 21–37 (30.7 ± 4.3) | - | No significant correlation between age and ADSC yield | - |
| Geissler et al. 2014 [27] | 2 | 24 | Lipoaspirate from healthy women. Multiple donor sites | 100% | 25–71 (51) | ≤45 (9) ≥ 46 (15) |
Analyses stratified by donor site: Abdomen: younger women showed significantly higher yield Flanks: older women had significantly higher ADSC yield | - |
| Aust et al. 2004 [28] | 2 | 18 | Elective liposuction | 87.5% | 20.5–29.3 (24.9 ± 2.7) | - | No significant correlation between age and ADSC yield | - |
| Schipper et al. 2008 [33] | 2 | 12 | Lipoaspirate from body-contouring surgery in previously obese patients | 100% | 25–60 | 25–30 (3) 40–45 (4) 55–60 (5) |
Higher proliferation of ADSC and lower susceptibility to apoptosis in 20 year old group (not statistically significant) | |
| Yoshimura et al. 2006 [35] | 2 | - | - | 100% | 21–59 | - | No significant correlation between ADSC yield and age | - |
| Zhu et al. 2009 [43] | 2 | - | Lipoaspirate from multiple donor sites | 100% | 20–58 | - | - | Slower rate of proliferation with increasing age (not statistically significant) Significant decrease in osteogenic potential |
ADSC adipocyte-derived stem cell, F female, LOE Level of Evidence, n number, SD standard deviation