Table 2.
Concentrations of cytokines (IL-6, IL-1 and TNF-α), acute phase proteins (CRP, Haptoglobin) and cells in different bodily fluids from control and infected animals
| Control (n = 4)a | Infected (n = 6, c n = 3) | |
|---|---|---|
| BALF Cytokines: | ||
| IL-6 (pg/ml) | ND | 434.2 (ND-2491.5) |
| IL-1β (pg/ml) | ND | 1166.8 (ND-2808.1) |
| TNF-α (pg/ml) | 129.5 (ND-410.4) | ND |
| BALF acute phase proteins: | ||
| CRP (ng/ml) | 89.7 (39.5–189.6) | 389.7 (192.6–554.4) |
| Hp (μg/ml) | 6.7 (5.2–9.1) | 6.2 (5.1–7.5) |
| BALF Cells: | ||
| Leukocytes (×109/l) | 2.9 (1.8–5.1) | 11.4 (7.8–16.9)** |
| Lymphocytes (×109/l) | 0.1 (0–0.3) | c2.5 (0.8–6.1) |
| Alveolar Macrophage (×109/l) | 1.7 (1.5–2.3) | c6.2 (4.5–10.6) |
| Infected (pre-infection)b | Infected (8 hpi) | |
| Serum acute phase proteins: | ||
| CRP (μg/ml) | 49.7 (18.4–71.5) | 202.2 (93.9–309)* |
| Hp (mg/ml) | 0.7 (0.2–1.1) | 0.7 (0.5–1.3) |
| Saliva acute phase proteins: | ||
| CRP (ng/ml) | 3 (1.3–7.4) | 27.9 (8.4–37.6) |
| Hp (μg/ml) | 0.4 (0.2–1) | 1.9 (1.3–3.6)* |
Results are expressed as median values (interquartile range). aDifferences between control and infected group in the BALF immediately after death. *p ≤ 0.05
**p ≤ 0.01 Wilcoxon rank test. bDifferences within the infected group between the last time point of sampling (8 hpi) and prior to infection. *p ≤ 0.05 Wilcoxon signed ranks test. cLymphocytes and alveolar macrophages were not detectable in the BALF of three animals out of the infection group
ND = not detectable because below detection limit
Significant values are marked in bold