Fig. 3.

Netrin-1 promotes angiogenesis in HUVEC. a Representative images of HUVEC tubule assay. Cells were exposed for 4 h to endothelial basal media (EBM), endothelial growth media (EGM), IgG (internal antibody control, 2 μg/mL), 2F5 [a drug targeting Netrin-1 (NTN-1), 2 μg/mL] and anti-NTN-1 antibody (R&D Systems, 2 μg/mL), in absence or presence of recombinant human NTN-1 (10 ng/mL), scale bar = 15 μm. b Quantified data correspond to the mean ± S.E.M. (n = 4, ^* p < 0.05 vs. EBM, ^γ p < 0.05 vs. NTN-1, ^α p < 0.05 vs. IgG + NTN-1)