Table 2.
Immunohistological analysis of tumors
| Therapeutic group | Necrosis (%) | Apoptosis (%) | Hypoxia (%) | Proliferation (%) | Vascularization (n of structures) | Immune cells (n of cells) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AM | SEM | AM | SEM | AM | SEM | AM | SEM | AM | SEM | AM | SEM | |||||||
| CTRL | 25.0 | ± | 2.6 | 11.3 | ± | 1.6 | 4.7 | ± | 1.0 | 92.2 | ± | 1.3 | 51.6 | ± | 3.2 | 7.9 | ± | 0.9 |
| pControl | 20.0 | ± | 2.6 | 11.6 | ± | 1.0 | 5.4 | ± | 1.0 | 92.1 | ± | 1.2 | 47.1 | ± | 4.7 | 8.3 | ± | 1.1 |
| TS | 21.7 | ± | 2.6 | 11.4 | ± | 1.5 | 5.5 | ± | 0.7 | 92.7 | ± | 1.0 | 50.5 | ± | 4.2 | 7.9 | ± | 0.7 |
| 3 × EP | 13.3 | ± | 3.6 | 18.7 | ± | 2.7 | 7.8 | ± | 0.8 | 74.4 | ± | 1.7 | 39.9 | ± | 2.6 | 14.3 | ± | 1.7 |
| 3 × GET (pControl) | 53.3 | ± | 7.9 | 19.5 | ± | 3.0 | 15.3 | ± | 2.9 | 75.6 | ± | 2.4 | 35.3 | ± | 4.2 | 15.1 | ± | 1.6 |
| 3 × GET (TS) | 65.8 | ± | 7.6 | 26.7 | ± | 2.2 | 46.3 | ± | 3.0 | 49.5 | ± | 3.8 | 14.9 | ± | 1.1 | 19.3 | ± | 1.7 |
| IR | 21.7 | ± | 4.2 | 18.4 | ± | 1.8 | 30.2 | ± | 2.4 | 53.3 | ± | 3.5 | 30.1 | ± | 2.3 | 26.8 | ± | 2.6 |
| pControl + IR | 37.5 | ± | 8.0 | 18.7 | ± | 1.9 | 32.3 | ± | 2.5 | 54.5 | ± | 4.7 | 24.5 | ± | 1.8 | 26.4 | ± | 2.1 |
| TS + IR | 43.3 | ± | 8.0 | 19.9 | ± | 1.5 | 31.6 | ± | 2.3 | 52.0 | ± | 2.9 | 23.8 | ± | 1.6 | 26.4 | ± | 2.3 |
| 3 × EP + IR | 25.0 | ± | 4.8 | 31.0 | ± | 2.0 | 51.7 | ± | 2.6 | 59.4 | ± | 4.0 | 28.9 | ± | 2.3 | 29.3 | ± | 2.1 |
| 3 × GET (pControl) + IR | 65.0 | ± | 7.6 | 38.7 | ± | 3.1 | 58.0 | ± | 2.8 | 46.0 | ± | 4.8 | 18.9 | ± | 2.0 | 45.8 | ± | 3.0 |
| 3 × GET (TS) + IR | 79.2 | ± | 4.2 | 50.3 | ± | 2.4 | 66.7 | ± | 1.6 | 46.7 | ± | 4.8 | 12.8 | ± | 1.0 | 46.5 | ± | 2.1 |
AM = Arithmetic mean; Groups: (CTRL = control; EP = electric pulses; GET = gene electrotransfer; IR = irradiation; TS = pET-antiCD105); n = Number of structures or cells; SEM = Standard error