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Double immunofluorescent staining for TRPM8 and VGLUT1 (A) or VGLUT2 (B), and size distribution (C) of TRPM8+ neurons and VGLUT2+/TRPM8+ neurons in the trigeminal ganglion. Many TRPM8+ neurons coexpress VGLUT2 (B, arrowheads). Note that the TRPM8+ neurons and VGLUT2+/TRPM8+ neurons are mostly small- and medium-sized. n = number of neurons. Scale bar = 50 µm.
