Fig. 8.
Double immunofluorescent staining for TRPM8 and VGLUT2 (A, B) and proportion (%) of TRPM8+, VGLUT1+, VGLUT2+ and VGLUT2+/TRPM8+ neurons (C) in the TG in the control vs. the CFA-treated mice. (A, B) The number of VGLUT2+/TRPM8+ neurons (arrowheads) increases significantly in the CFA-treated group compared to control. (C) The proportion (Mean ± SE) of VGLUT2+/TRPM8+ neurons, but not of VGLUT1+/TRPM8+ neurons, is significantly higher in the CFA-treated group compared to control. N = 5 animals per group. *p < 0.05. Scale bar = 50 µm.