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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Nature. 2017 Feb 15;542(7642):450–455. doi: 10.1038/nature21365

Figure 5. BAT-derived exosomes expressing human miRNA miR-302f target their reporter in liver in vivo.

Figure 5

(a) Protocol 1. Schematic of in vivo targeting protocol using adenovirus bearing pre-miR-302f or LacZ directly into BAT. (b) C57Bl/6 mice injected i.v. with pacAd5-hsa_miR-302f 3’-UTR reporter after BAT injection of Ad-pre-hsa-miR-302f or Ad-LacZ subjected to IVIS (n=4 per group). (c) Total flux luminescence obtained via IVIS analysis from mice in Panel B. (n=4/group, p=0.028, Mann-Whitney U-test). (d) Protocol 2. Schematic of in vivo targeting protocol injecting exosomes from C57Bl/6 mice transduced with pre-miR-302f or adenovirus bearing LacZ directly into BAT. (e) C57Bl/6 mice transduced with pacAd5-hsa_miR-302f 3’-UTR reporter after i.v. injections of serum exosomes from Ad-pre-hsa_miR-302f or Ad-LacZ BAT injected mice and subjected to IVIS analysis (n=4 per group). (f) Total flux luminescence obtained from using protocol in panel e (n=4/group, p=0.028, two-tailed Mann-Whitney U-test). Bars represent SEM. (g) Model of mechanisms by which fat-derived circulating exosomal miRNAs might regulate target mRNAs in other tissues.