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. Author manuscript; available in PMC: 2018 Feb 8.
Published in final edited form as: Neuron. 2017 Jan 19;93(3):606–615.e3. doi: 10.1016/j.neuron.2016.12.020

Figure 4. Glut4 is required for synaptic vesicle recycling following bursts of AP firing.

Figure 4

(A-D) Sample vGlut-pH traces in response to 100 AP (10 Hz) in (A) control, Glut4 KD, and Glut4 KD neurons expressing shRNA-resistant Glut4-RFP, or (B) in Glut4 KD neurons where stimulation was followed by quenching of extracellular pHluorin with MES acid. (C) Average endocytic block measured as the fraction of vGlut-pH signal remaining at two endocytic time constants (2⊺) of the control at the end of stimulation. Control: 0.15 ± 0.03, Glut4 KD: 0.7 ± 0.1, rescue: 0.26 ± 0.07; n = 13-17 cells. (D) Average exocytosis of vGlut-pH measured as ΔF at the end of 100 AP normalized to ΔFNH4Cl (% max). Control: 23 ± 4, Glut4 KD: 25 ± 4; n= 13-15 cells. All error bars are SEM. **** P<0.0001. See also Figure S4.