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. Author manuscript; available in PMC: 2017 Feb 28.
Published in final edited form as: FEBS J. 2009 Apr;276(8):2348–2358. doi: 10.1111/j.1742-4658.2009.06967.x

Fig 2.

Fig 2

Inhibition of adipogenic differentiation by miR-27. 3T3-L1 preadipocytes were grown to confluence and transfected with equal total amounts of each of the following miRNA molecules: miR-27a, miR-27b, miR-27a/miR-27b (1:1), miR control (Ctrl). Adipogenic differentiation was initiated at 24 hr post-transfection. Cells were fixed and stained with Oil Red O on Day 6 of differentiation (A). The amount of Oil Red O was quantified after extraction with isopropanol. Data shown in (B) are mean ± sem of a triplicate experiment. For the time course study, miRNA transfection was indicated in relationship to the start of the IDM-treatment at Day 0. (C). Cells were fixed and stained with Oil Red O on Day 4 of differentiation (D). Quantification of Oil Red O was shown in (E). Positive = differentiated L1 cells without miRNA transfection. Negative = undifferentiated 3T3-L1 cells. The results shown were confirmed by more than three independent experiments.