Fig. 3.

Representative confocal images of ICC labeling for VGLUT2, GLP1, and PHAL in the vlBST. a 5-μm-thick projection image demonstrates virtually all GLP1-ir varicose axons/buttons are VGLUT2-ir; inserted ×10 epifluorescence image shows the distribution of GLP1-ir axon terminals in the vlBST and the area (hash) where confocal image was taken. b 5-μm-thick projection image demonstrates the localization of VGLUT2-ir in NST-origin PHAL-ir varicose axon terminals. 3D rotated inserts (a, b) are maximum intensity projection from the regions of interest