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. 2004 Oct 28;23(23):4670–4678. doi: 10.1038/sj.emboj.7600452

Figure 5.

Figure 5

Stabilization of BAF/DNA complex by LAP2α in vitro. (A) Retention of intermolecular integration activity of MoMLV PICs after treatment with 400 mM KCl. The PIC fractions were incubated in the presence of 150 mM (lane 1), 400 mM (lane 2), or 750 mM (lane 3) KCl and, after gel filtration, samples were added to integration reaction mixtures containing Φ × 174 RFI DNA. Products were digested with BamHI and detected by Southern blotting. (B) Velocity sedimentation of BAF/DNA complexes with or without LAP2α. BAF and LAP2α were incubated with Φ × 174 DNA in buffer containing 115 mM KCl as indicated and then challenged with 150 or 400 mM KCl. After centrifugation in a sucrose gradient containing the same concentration of KCl, gradients were fractionated and the DNA was detected by Southern blotting.