Figure 3.
Compound 3 does not directly inhibit the formation of glycolipid C. (A) The trypanosome cell-free system was pulse-labelled with GDP-[3H]Man, to form labelled glycolipids A′ and θ, and then chased with cold GDP-Man and a fatty-acid remodelling mix. The remodelled (glycolipid A) and inositol acylated (glycolipid C) products were extracted and analysed by HPTLC and by scanning with a linear analyser. (B) As for (A), except that PMSF was added to the chase. (C) As for (A), except that compound 3 was added to the chase.