Table 1.
Characterization of the [3H]mannosylated glycolipids formed in the trypanosomal cell-free system with various GlcN-PI analogues
| Abbreviationsa | JBαMb | PI-PLC | Basec | Glycan sized | Assignment |
|---|---|---|---|---|---|
| M1 | +e | + | + | 2.3 | Man1GlcN-PI |
| M2 | + | + | + | 3.1 | Man2GlcN-PI |
| M3 | + | + | + | 4.2 | Man3GlcN-PI |
| aM3 | + | − | + | 4.1 | Man3GlcN-(acyl)PI |
| C′ | − | − | + | 4.1 | EtNP-Man3GlcN-(acyl)PI |
| A′ | − | + | + | 4.1 | EtNP-Man3GlcN-PI |
| C | − | − | + | 4.2 | EtNP-Man3GlcN-(acyl)PI(dimyristyl) |
| A | − | + | + | 4.2 | EtNP-Man3GlcN-PI(dimyristyl) |
| M1-GlcN(2-O-Me)I-P-C18) | + | − | − | 2.4 | Man1-GlcN(2-O-Me)I-P-C18) |
| M2-GlcN(2-O-Me)I-P-C18) | + | − | − | 3.2 | Man2-GlcN(2-O-Me)I-P-C18) |
| M3-GlcN(2-O-Me)I-P-C18) | + | − | − | 4.2 | Man3-GlcN(2-O-Me)I-P-C18) |
| aM1-GlcN(2-O-Me)I-P-C18) | + | − | +(M1-GlcN(2-O-Me)I-P-C18) | 2.3 | Man1-GlcN(2-O-Me)(acyl)I-P-C18) |
| aM2-GlcN(2-O-Me)I-P-C18) | + | − | +(M2-GlcN(2-O-Me)I-P-C18) | 3.1 | Man2-GlcN(2-O-Me)(acyl)I-P-C18) |
| aM3-GlcN(2-O-Me)I-P-C18) | + | − | +(M3-GlcN(2-O-Me)I-P-C18) | 4.3 | Man3-GlcN(2-O-Me)(acyl)I-P-C18) |
| See Figures 1 and 2 for corresponding bands. All of the glycolipids listed here were sensitive to GPI-PLD and deamination. | |||||
| JBαM=jack bean α-mannosidase. | |||||
| The products of base treatment, if sensitive, are given in brackets. | |||||
| The size of the neutral glycans terminating in 2,5-anhydromannitol (AHM) were determined as described in Materials and methods, and are expressed in glucose units. The published values for authentic Manα1–4AHM, Manα1–6Manα1–4AHM and Manα1–2Manα1–6Manα1–4AHM are 2.4, 3.2 and 4.2 glucose units, respectively (Ferguson, 1992). | |||||
| ‘+' indicates a positive digestion, that is, a loss (or shift) of the radiolabelled band; ‘−' indicates a negative digestion, that is, no loss or shift of band. | |||||